5 steps of gram staining

Hans Christian Gram, the inventor of the Gram staining technique, was a pioneering biologist who devised the system of classification which led to as many as 30,000 formally named species of bacteria being investigated.

He’s the subject of the latest Google doodle, created to honour his birth date of 13 September 1853. Gram, working with German pathologist and microbiologist Carl Friedlander, devised the technique in Berlin in the early 1880s.

Gram-variable

Gram staining procedure in flowchart

Gram staining introduction

Gram staining researchgate

Gram staining procedure

It is still known as one of the most important staining techniques used in microbiology to identify bacteria under a microscope.

Gram first dripped reagents, a substance designed to cause a chemical reaction, onto lung tissue samples.

He found differences in the colouring of bacteria that is now known to be Streptococcus pneumoniae and Klebsiella pneumoniae. The differences Gram observed are a result of the composition of the bacterial cell wall. Some bacteria have a cell